Protein_Domain
Part:BBa_K1935018:Design
Designed by: Emilie Gounin Group: iGEM16_Bordeaux (2016-10-14)
dead Cas9 (dCas9)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1078
Illegal NgoMIV site found at 2182
Illegal NgoMIV site found at 2255
Illegal NgoMIV site found at 2740
Illegal NgoMIV site found at 3649 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutagenesis was done to abolish illegal restriction sites.
Pst1 restriction sites : 2214 pb, 2418 pb and 2448 pb (G => C)
Source
https://www.addgene.org/47106/sequences/